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The mitochondrial genome sequence analysis of Ophidascaris baylisi from the Burmese python (Python molurus
Qi Zhao1, Asmaa M I Abuzeid1, Long He1
1Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510542, China.
Parasitology International
|August 10, 2021
Summary
This study identifies Ophidascaris baylisi, a parasitic roundworm from Burmese pythons, using its mitochondrial genome. This research clarifies the classification of Ophidascaris nematodes and provides new molecular markers.
Area of Science:
- Parasitology
- Molecular Biology
- Genomics
Background:
- Ophidascaris species are parasitic roundworms found in python guts, causing significant health issues.
- The classification and nomenclature of these snake roundworms remain subjects of debate.
- Accurate identification and genetic characterization are crucial for understanding their biology and impact.
Purpose of the Study:
- To identify a specific Ophidascaris species from the Burmese python (Python molurus bivittatus).
- To analyze the complete mitochondrial genome of the identified roundworm.
- To clarify the taxonomic and phylogenetic relationships within the Ophidascaris genus.
Main Methods:
- Morphological analysis of the parasitic roundworm.
- Sequencing of the cytochrome c oxidase subunit I (cox1) gene for identification.
- Whole mitochondrial genome sequencing and analysis.
- Phylogenetic analysis using mitochondrial protein-coding genes.
Main Results:
- The roundworm was identified as Ophidascaris baylisi based on morphology and cox1 sequence.
- The complete mitochondrial genome of O. baylisi is 14,784 bp, containing 36 genes and two non-coding regions.
- Phylogenetic analysis placed O. baylisi within the Ascarididae family, closely related to Ophidascaris wangi.
Conclusions:
- This study provides a definitive identification of Ophidascaris baylisi and its complete mitochondrial genome.
- The findings contribute to the nematode mitochondrial genome database, enhancing taxonomic and phylogenetic understanding.
- The molecular data offer valuable markers for future research on Ophidascaris nematodes.
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