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Related Experiment Video

Updated: Oct 25, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
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The developing mouse coronal suture at single-cell resolution.

D'Juan T Farmer1, Hana Mlcochova2, Yan Zhou2

  • 1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, USA.

Nature Communications
|August 11, 2021
PubMed
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This summary is machine-generated.

This study reveals distinct cell types within the embryonic coronal suture, identifying a key Six2+ osteoprogenitor population. These cells are crucial for skull development and are implicated in craniosynostosis.

Area of Science:

  • Developmental Biology
  • Craniofacial Development
  • Single-cell Genomics

Background:

  • Skull sutures are essential for brain growth and cranial development.
  • The coronal suture's development and role in craniosynostosis are not fully understood.
  • Monogenic craniosynostosis often involves premature fusion of the coronal suture.

Purpose of the Study:

  • To characterize the cellular diversity of the murine embryonic coronal suture.
  • To identify progenitor populations involved in coronal suture development.
  • To investigate the cellular basis of craniosynostosis in a mouse model.

Main Methods:

  • Single-cell RNA sequencing (scRNA-seq) of murine embryonic coronal sutures.
  • Expression validation using various techniques.

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  • Lineage tracing experiments.
  • Analysis of a Twist1+/-; Tcf12+/- mouse model.

    • Main Results:

    • Identification of distinct pre-osteoblast signatures in bone fronts and periosteum.
    • Discovery of a persistent ligament-like suture mesenchyme and a dura mater chondrogenic-like population.
    • Characterization of an embryonic Six2+ osteoprogenitor population contributing to postnatal suture mesenchyme.
    • Demonstration that Six2+ progenitors are affected in a Saethre-Chotzen Syndrome mouse model.

    Conclusions:

    • The murine coronal suture comprises diverse cell populations with specific roles.
    • Six2+ osteoprogenitors are critical for suture development and are implicated in craniosynostosis.
    • This single-cell atlas serves as a valuable resource for studying coronal suture development and craniosynostosis.