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A Novel miRNA-mRNA Axis Involves in Regulating Transcriptional Disorders in Pancreatic Adenocarcinoma

Xin Shang1, Lan-Er Shi1, Dina Taule1

  • 1The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, People's Republic of China.

Cancer Management and Research
|August 11, 2021
PubMed
Abstract

Insights

Researchers identified a novel microRNA (miRNA)-messenger RNA (mRNA) network in pancreatic adenocarcinoma (PAAD). This discovery offers a potential new strategy for treating PAAD by targeting transcriptional dysregulation and patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background:

  • Pancreatic adenocarcinoma (PAAD) mechanisms and therapeutic targets remain poorly understood.
  • The role of microRNA (miRNA)-messenger RNA (mRNA) networks in PAAD pathogenesis is largely unexplored.

Purpose of the Study:

  • To investigate the potential of miRNA-mRNA networks in identifying regulatory mechanisms and therapeutic targets for PAAD.
  • To construct a novel miRNA-hub gene regulatory network for PAAD.

Main Methods:

  • Differential expression analysis of miRNAs and genes in PAAD tissues using GEO data.
  • Identification of transcription factors, hub genes, and key miRNAs.
  • Pathway enrichment analysis (GO, KEGG) and survival analysis (UALCAN, Kaplan-Meier).
  • Validation of candidate miRNA-mRNA interactions using qRT-PCR.

Main Results:

  • Identified 62 differentially expressed miRNAs (DEMIs) and 2424 differentially expressed genes (DEGs).
  • Constructed a novel miRNA-hub gene regulatory network, highlighting 8 key miRNAs.
  • Found decreased miR-455-3p expression correlated with poor prognosis in PAAD.
  • Validated HOXC4, DLG4, DYNLL1, and FBXO45 as probable targets of miR-455-3p.

Conclusions:

  • A novel miRNA-mRNA axis (miR-455-3p and its targets) is implicated in PAAD transcriptional dysregulation and survival.
  • This finding provides a potential novel therapeutic strategy for PAAD treatment.
Keywords:
bioinformatics analysisdifferentially expressed genesdifferentially expressed microRNAshsa-miR-455-3ppancreatic adenocarcinoma

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