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Propofol Suppresses Gastric Cancer Progression by Regulating circPDSS1/miR-1324/SOX4 Axis

Leyi Liu1, Ting Dong2, Jun Sheng3

  • 1Department of Anesthesiology, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, 241000, People's Republic of China.

Cancer Management and Research
|August 11, 2021
PubMed
Abstract

Insights

Propofol, an anesthetic, shows anticancer effects in gastric cancer (GC) by regulating the circPDSS1/miR-1324/SOX4 pathway. This suggests propofol

Area of Science:

  • Oncology
  • Molecular Biology
  • Anesthesiology

Background:

  • Propofol, a common intravenous anesthetic, exhibits antitumor properties in various human cancers.
  • Circular RNAs (circRNAs) are implicated in cancer progression, but their role in gastric cancer (GC) concerning propofol remains unexplored.
  • This study investigates the novel relationship between propofol and circRNA decaprenyl diphosphate synthase subunit 1 (circPDSS1) in GC.

Purpose of the Study:

  • To elucidate the mechanism by which propofol exerts its antitumor effects in gastric cancer.
  • To investigate the role of circPDSS1, miR-1324, and SOX4 in propofol-mediated anticancer activity in GC.
  • To explore the potential of propofol as a therapeutic agent for GC treatment.

Main Methods:

  • Cell proliferation, migration, invasion, and apoptosis were assessed using CCK-8, colony formation, EdU, and flow cytometry assays.
  • Quantitative real-time PCR (qRT-PCR) and Western blot were employed to determine the expression levels of circPDSS1, miR-1324, and SOX4.
  • Dual-luciferase reporter and RNA pull-down assays confirmed the interactions between circPDSS1, miR-1324, and SOX4. In vivo efficacy was evaluated using a mice xenograft model.

Main Results:

  • Propofol inhibited GC cell proliferation, migration, and invasion while inducing apoptosis; these effects were reversed by upregulating circPDSS1.
  • CircPDSS1 acts as a molecular sponge for miR-1324, promoting GC progression and counteracting propofol's effects.
  • MiR-1324 directly targets SOX4, mediating the anticancer role of propofol. CircPDSS1 overexpression diminished propofol's in vivo anticancer efficacy.

Conclusions:

  • Propofol demonstrates significant anticancer activity in gastric cancer by modulating the circPDSS1/miR-1324/SOX4 molecular axis.
  • The findings suggest that propofol holds promise as an effective therapeutic agent for gastric cancer treatment.
  • Targeting the circPDSS1/miR-1324/SOX4 pathway could be a viable strategy for enhancing propofol-based GC therapy.
Keywords:
SOX4circPDSS1gastric cancermiR-1324propofol

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