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LINC00671 inhibits renal cell cancer progression via regulating miR-221-5p/SOCS1 axis

Gang Jin1, Haiyan Mi2, Yunfei Ye3

  • 1Department of Urology Surgery, The No. 1 People's Hospital of Pinghu Community Pinghu 314200, China.

American Journal of Translational Research
|August 11, 2021
PubMed
Abstract

Insights

Long non-coding RNA LINC00671 acts as a tumor suppressor in renal cell cancer (RCC). It inhibits cancer cell growth, migration, and invasion by regulating the miR-221-5p/SOCS1 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their regulatory roles in cancer progression.
  • The specific function of lncRNA LINC00671 in renal cell cancer (RCC) remains largely undetermined.
  • Understanding lncRNA roles is crucial for developing novel cancer therapies.

Purpose of the Study:

  • To investigate the role and mechanism of lncRNA LINC00671 in renal cell cancer (RCC).
  • To determine the relationship between LINC00671 expression and RCC clinicopathological features and patient survival.
  • To elucidate the molecular pathway regulated by LINC00671 in RCC progression.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) for gene expression analysis.
  • Western blotting to assess protein levels of epithelial-mesenchymal transition (EMT)-associated markers and SOCS1.
  • Cell proliferation, migration, invasion assays (CCK-8, EdU, Transwell), RNA immunoprecipitation (RIP), and luciferase reporter assays to confirm molecular interactions.

Main Results:

  • LINC00671 expression was significantly decreased in RCC tissues and cell lines, correlating with poorer survival and advanced clinicopathological features.
  • Overexpression of LINC00671 suppressed RCC cell proliferation, migration, invasion, and EMT in vitro.
  • LINC00671 was found to target miR-221-5p, thereby up-regulating suppressor of cytokine signaling 1 (SOCS1) expression.

Conclusions:

  • LINC00671 functions as a tumor suppressor in renal cell cancer (RCC).
  • The mechanism involves the regulation of the miR-221-5p/SOCS1 axis.
  • LINC00671 may serve as a potential therapeutic target for RCC treatment.
Keywords:
LINC00671MiR-221-5pRCCSOCS1

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