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Updated: Oct 25, 2025

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

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miR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression

Yanjing Jia1, Jie Zhao2, Jinjie Yang3

  • 1Department of General Surgery, Huashan Hospital, Fudan University, Shanghai 200040, PR China.

Molecular Therapy Oncolytics
|August 11, 2021
PubMed

Insights

MicroRNAs (miRNAs) like miR-301 promote breast cancer progression by inhibiting CPEB1, which regulates the SIRT1/SOX2 pathway. This accelerates tumor growth and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading global health concern.
  • MicroRNAs (miRNAs) are key regulators of gene expression in cancer.
  • Identifying specific miRNAs and their roles is crucial for understanding breast cancer progression.

Purpose of the Study:

  • To identify specific miRNAs involved in breast cancer progression.
  • To elucidate the molecular mechanisms underlying miR-301's role in breast cancer.
  • To investigate the functional impact of miR-301 and its downstream targets on malignant phenotypes.

Main Methods:

  • Bioinformatics analysis to identify differentially expressed miRNAs.
  • Expression determination and functional assays (luciferase, RIP) to study miRNA-target interactions.
  • In vivo studies using nude mice to assess tumorigenicity.

Main Results:

  • miR-301 was found to be overexpressed in breast cancer.
  • miR-301 overexpression inhibited cytoplasmic polyadenylation element-binding protein 1 (CPEB1) levels.
  • miR-301 promoted cell proliferation, metastasis, cell cycle progression, and impeded apoptosis, while accelerating tumor formation in vivo.
  • CPEB1 mediated breast cancer development via the SIRT1/SOX2 pathway.

Conclusions:

  • miR-301 overexpression accelerates breast cancer progression.
  • The miR-301/CPEB1/SIRT1/SOX2 axis is a critical pathway in breast cancer development.
  • miR-301 represents a potential therapeutic target for breast cancer treatment.
Keywords:
CPEB1SIRT1/SOX2 pathwayapoptosisbreast cancerinvasionmiR-301migrationproliferation

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