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Updated: Oct 25, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Characterization of the complete mitochondrial genome of Neolissochilus hexastichus (McClelland, 1839)
Shubra Singh1, Archana Mishra1, Annam Pavan-Kumar1
1Division of Fish Genetics and Biotechnology, ICAR - Central Institute of Fisheries Education Versova, Mumbai, India.
Abstract:
The complete mitochondrial genome of the near threatened mahseer fish, Neolissochilus hexastichus, was characterized for the first-time using Ion Torrent NGS platform. The total length of the mitogenome was 16,538 bp including a standard set of 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs), 13 protein-coding genes and a non-coding control region. Twenty-eight of the 37 genes are located on the light strand and, the remaining nine genes are situated on the heavy strand. Phylogenetic analysis showed the sister relationship between N. hexastichus and N. hexagonolepis. The mitogenome could be useful for phylogenetics, population genetics, and conservation of the mahseers.
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