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Adenovirus aggregation and preservation in extracellular environment
Archives of Virology
|January 1, 1979
Summary
Adenovirus aggregation is influenced by ionic strength, pH, particle concentration, and temperature. Optimal conditions for adenovirus type 2 aggregation were identified, with reversibility noted at specific pH levels.
Area of Science:
- Virology
- Biophysics
Background:
- Adenovirus aggregation can impact viral infectivity and stability.
- Understanding aggregation conditions is crucial for viral research and applications.
Purpose of the Study:
- To analyze the conditions affecting adenovirus type 2 aggregation.
- To determine the influence of ionic strength, pH, particle concentration, and temperature on virion aggregation.
Main Methods:
- Adenovirus type 2 was propagated on Hep2 cells.
- Virion aggregation was analyzed using sedimentation velocity on sucrose gradients.
Main Results:
- Aggregation occurred between 0.005 M and 0.05 M NaCl.
- At pH 4-5, 90% of particles aggregated, with irreversibility below pH 4.
- Aggregation decreased with dilution and below 20 degrees C, with no aggregation at +4 C.
- The aggregation reaction showed a positive deltaH (17.9 Kcal/mol) and negative deltaG (-1.32 Kcal/mol).
Conclusions:
- Adenovirus type 2 aggregation is highly sensitive to environmental factors.
- Specific ionic strength, pH, concentration, and temperature ranges promote or inhibit adenovirus aggregation.