Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association of small dense LDL cholesterol and inflammation with plaque rupture in acute coronary syndrome: An OCT study.

Journal of clinical lipidology·2026

Same author

Cold Agglutinin Disease-Like Genetic Signatures in Lymphoplasmacytic Lymphoma-Like Cases Challenge the WHO Fifth Edition Classification.

EJHaem·2026

Same author

Reduction in hippocampal cholinergic neurostimulating peptide enhances memory impairment in App<sup>NL-G-F</sup> KI mice.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Same author

A low temperature-adapted <i>Euglena gracilis</i> ecotype for outdoor biomass production in colder climates.

Applied and environmental microbiology·2026

Same author

Association of the residual SYNTAX score on clinical outcomes in patients with acute coronary syndrome.

Atherosclerosis·2026

Same author

Enlarged perivascular spaces in patients with lacunar infarctions.

Current neurovascular research·2026

Search research articles

Related Experiment Video

Updated: Oct 25, 2025

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

Microscopic impedance cytometry for quantifying single cell shape.

Tao Tang¹, Xun Liu¹, Ryota Kiya¹

¹Division of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama-cho Ikoma, Nara, 630-0192, Japan.

Biosensors & Bioelectronics

|August 11, 2021

Summary

This study introduces a new "tilt index" to measure cell shape using impedance flow cytometry. This method accurately distinguishes between symmetrical and asymmetrical cells without needing microscopy.

Keywords:

Impedance flow cytometry Microfluidics Shape characterization Single-cell analysis

More Related Videos

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Related Experiment Videos

Last Updated: Oct 25, 2025

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Published on: June 24, 2020

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Area of Science:

Biophysics
Cell Biology
Analytical Chemistry

Background:

Impedance flow cytometry is a powerful technique for analyzing cells and particles.
Characterizing the shape of single cells is crucial for understanding their function and behavior.
Existing methods for shape analysis often rely on complex optical setups.

Purpose of the Study:

To investigate the capability of impedance flow cytometry in determining the morphology of individual cells/particles.
To develop a novel metric for quantifying cell shape based on impedance pulse characteristics.
To validate the new metric's effectiveness and independence from particle trajectory.

Main Methods:

Simulations were conducted to evaluate the proposed 'tilt index' metric.
Experiments utilized a custom-built lock-in amplifier for impedance measurements.
Polystyrene beads and Euglena gracilis cells were used as test subjects.

Main Results:

Asymmetrical micro-objects exhibited a distinct tilting trend in impedance pulses, unlike symmetrical ones.
The 'tilt index' was found to be zero for symmetrical objects and greater than zero for asymmetrical objects.
The 'tilt index' demonstrated independence from the micro-object's trajectory during measurement.

Conclusions:

The developed 'tilt index' metric enables accurate cell/particle shape characterization using impedance cytometry.
This approach offers a label-free and microscopy-free method for high-throughput cell shape analysis.
The findings open new avenues for non-optical cell morphology assessment in biological and material science applications.