Effect of gamma irradiation on growth, proline content, bioactive compound changes, and biological activity of 5...

Gamma irradiation is the technique used to induce plant mutation and it has affected both the physiological and biochemical compounds of the plant. Some new rice lines are also created through the gamma-irradiation technique. We investigated the effect of gamma irradiation on plant growth, volatile compounds, and the biological activity of gamma-irradiated rice extract compared to non-gamma-irradiated rice extract. The results reveal that the gamma-irradiated rice growth was related to the proline content, as the low gamma dose induced rice growth and proline accumulation in gamma-irradiated rice. We induced the bioactive compounds, including the flavonoid content and phenolic content of gamma-irradiated rice, through the low gamma irradiation dose at 60-100 Gy. Interestingly, bioactive compounds were stimulated by a gamma dose similar to that of the biological activity (antioxidant activity and enzyme inhibition) of gamma-irradiated rice. The results suggest that gamma-irradiated rice extract's biological activity was closely related to the flavonoid and phenolic content of rice. We also identified the variety of volatile compounds in gamma-irradiated rice and they were also reported for the biological activity. Our results can generate a new rice line that exhibits high plant growth and is rich with bioactive compounds such as flavonoid and phenolic compounds which are related to the improvement of human health.