Related Experiment Video
Updated: Oct 24, 2025
A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
Published on: June 17, 2021
Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron
Michael W Martynowycz1,2, Tamir Gonen1,2,3
1Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Abstract:
We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).
