Protocols for studying bacteriophage interactions with in vitro epithelial cell layers
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Updated: Oct 24, 2025

Author Spotlight: Investigating Bacteriophage-Induced Immune Responses in Gnotobiotic Mice
Published on: January 26, 2024
Marion C Bichet1, Ruzeen Patwa1, Jeremy J Barr1
1School of Biological Sciences, Monash University, Clayton Campus, Melbourne, VIC 3800, Australia.
Abstract:
Interactions between bacteriophages and mammalian cells are poorly understood. Establishing common methodologies investigating these interactions is important for advancing our understanding in this area. The protocols presented here provide an overview of key approaches investigating interactions between bacteriophages and eukaryotic cells using a variety of techniques, including transwells, microscopy, and whole-cell analysis. These techniques allow for the direct measurement of phage-cellular interactions and characterization of how the presence of phages affects cellular pathways, cell biology, immunology, and the microbiome. For complete details on the use and execution of this protocol, please refer to Nguyen et al. (2017) and Bichet et al. (2021).
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