Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Durum wheat under plastic pressure: genotype-specific adaptation to polystyrene nanoplastics.

Plant physiology and biochemistry : PPB·2026

Same author

Recommendations of the Polish Zebrafish Society on the use of the zebrafish (<i>Danio rerio</i>) model in biomedical research.

Acta biochimica Polonica·2026

Same author

Immunothrombosis promotes the interaction between neutrophils and synovial fibroblasts in rheumatoid arthritis.

Translational research : the journal of laboratory and clinical medicine·2026

Same author

Genomic characterization and preclinical evaluation of the candidate probiotic strain <i>Lactococcus cremoris</i> FBMS_5810.

Frontiers in microbiology·2026

Same author

Chromatin dynamics of the Klf4 locus in mouse pluripotent cells.

Scientific reports·2026

Same author

Metabolic alterations driven by PFKFB3 upregulation confer resistance to trastuzumab in HER2-positive breast cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Search research articles

Related Experiment Video

Updated: Oct 24, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Low Input Targeted Chromatin Capture (Low-T2C).

Ilias Boltsis¹, Karol Nowosad^1,2,3, Rutger W W Brouwer^1,4

¹Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)

|August 12, 2021

Summary

This summary is machine-generated.

This study details the low-input targeted chromatin capture (low-T2C) protocol, an optimized method for analyzing 3D chromatin organization in small cell populations. It provides a comprehensive guide to experimental steps and bioinformatics for studying gene regulation and cellular processes.

Keywords:

C-technologies Chromatin Chromatin conformation capture Genome architecture T2C Three-dimensional (3D) organization Topologically associating domains (TADs)

More Related Videos

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Related Experiment Videos

Last Updated: Oct 24, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Area of Science:

Genomics
Molecular Biology
Epigenetics

Background:

3D chromatin organization is crucial for gene regulation, cell cycle progression, and development.
Conventional methods like Targeted Chromatin Capture (T2C) require substantial cell numbers.
Analyzing chromatin structure in limited cell samples presents a significant technical challenge.

Purpose of the Study:

To present a detailed protocol for low-input targeted chromatin capture (low-T2C).
To enable the study of 3D chromatin organization in low cell number samples.
To provide a comprehensive resource for researchers using low-T2C.

Main Methods:

Optimization of the Targeted Chromatin Capture (T2C) protocol for reduced cell input.
Detailed description of experimental procedures for low-T2C.
Inclusion of essential bioinformatics tools for data analysis.

Main Results:

The low-T2C protocol is effectively described in detail.
The protocol is suitable for analyzing chromatin organization with limited cell samples.
Associated bioinformatics workflows are outlined for practical application.

Conclusions:

The low-T2C protocol offers a valuable method for investigating 3D genome architecture in scarce cell populations.
This detailed protocol facilitates the study of chromatin dynamics in various biological contexts.
Researchers can now explore chromatin interactions with greater flexibility using low cell numbers.