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Purification and characterization of a lectin from Phaseolus acutifolius var. latifolius
F Vargas-Albores1, G de la Fuente, C Agundis
1Unidad de Medicina Experimental, Facultad de Medicina, C.U., Universidad Nacional Autónoma de México, Cuernavaca, Morelos.
Preparative Biochemistry
|January 1, 1987
Abstract:
The lectin from Phaseolus acutifolius var. latifolius has been purified to homogeneity by affinity chromatography using stroma-sephadex, gel filtration and electrofocusing. The purified lectin consists of four subunits of 21 Kd molecular mass each one with 7.4% w/w total carbohydrate. Red cells of human and animal species, are agglutinated but are not inhibited by a series of sugars. Mitogenicity and immunosuppressive activities were demonstrated. Agglutination requires magnesium and calcium ions.