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Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition
Jun-Hyeok Yu1,2, Do-Won Park1, Jeong-A Lim3
1Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 13120, Korea.
Journal of Microbiology (Seoul, Korea)
|August 12, 2021
Summary
This study characterizes LysSAP33, a novel staphylococcal endolysin with broad antimicrobial activity. LysSAP33 demonstrates potent efficacy against Staphylococcus biofilms, offering a promising alternative to traditional antibiotics.
Area of Science:
- Microbiology
- Biotechnology
- Antimicrobial Research
Background:
- Bacteriophage endolysins are promising antimicrobial agents against Staphylococcus species.
- LysK-like endolysins are well-studied, but non-LysK-like endolysins remain underexplored.
- LysSAP33 is a novel endolysin from bacteriophage SAP33 with low homology to LysK-like endolysins.
Purpose of the Study:
- To characterize the novel staphylococcal endolysin LysSAP33.
- To investigate the role of its peptidoglycan-binding domain (CBD).
- To evaluate LysSAP33's lytic activity and efficacy against Staphylococcus biofilms compared to LysK-like endolysins.
Main Methods:
- Sequence and domain analysis of LysSAP33.
- Green fluorescence assay to confirm peptidoglycan binding of LysSAP33_CBD.
- Lytic activity assays under varying temperature, pH, and salt concentrations.
- Biofilm inhibition assays.
Main Results:
- The CBD domain of LysSAP33 (157-251 aa) binds to Staphylococcus aureus peptidoglycan.
- Deletion of LysSAP33_CBD significantly reduced lytic activity.
- LysSAP33 exhibited broader optimal conditions (temperature, pH, NaCl) than LysK-like endolysins.
- LysSAP33 showed superior activity against Staphylococcus biofilms.
Conclusions:
- LysSAP33 is a functional endolysin with a crucial CBD domain for activity.
- LysSAP33 offers enhanced stability and superior anti-biofilm properties compared to LysK-like endolysins.
- LysSAP33 represents a potential biocontrol agent for staphylococcal infections and biofilms.
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