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Updated: Aug 15, 2026

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
[Microcinematographic study of pancreatic islet cells from fetal cattle]
Abstract:
The growth characteristics of pancreatic islet cell cultures of bovine fetuses were investigated by the method of time-lapse cinemicrography. These monolayer cultures consisted of epithelial cells only. Under the influenc of high glucose concentration in the growth medium (up to 300 mg per 100 ml) there occurred an activation of mitochondrial apparatus of the islet cells, stimulation of cytogranulokinesis, and intensification of accumulation of the secretory granules with the subsequent degranulation of the islet cells. These data were compared with the results of cytological control of fixed and stained culture.

