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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

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Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
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Abnormal Proliferation02:23

Abnormal Proliferation

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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
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Related Experiment Video

Updated: Oct 24, 2025

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

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MicroRNA-20a Targeting LASS2 Promotes the Proliferation, Invasiveness and Migration of Bladder Cancer.

Shiwei Xiao, Yujin Chen, Ting Luan

    Clinical Laboratory
    |August 12, 2021
    PubMed
    Summary
    This summary is machine-generated.

    MicroRNA 20a (miR-20a) is upregulated in bladder cancer, promoting tumor progression and metastasis. It directly targets LASS2, indicating its oncogenic role in this malignancy.

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    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • MicroRNA 20a (miR-20a) exhibits varied roles in different cancers.
    • Its specific function in bladder cancer remains incompletely understood.

    Purpose of the Study:

    • To investigate the role and underlying mechanisms of miR-20a in bladder cancer.
    • To identify potential therapeutic targets related to miR-20a.

    Main Methods:

    • Quantitative real-time PCR (qRT-PCR) to measure miR-20a expression in 96 bladder cancer patients.
    • In vitro assays (wound healing, CCK8, transwell) to assess miR-20a's functional impact.
    • Bioinformatics and molecular assays (Western blot, qRT-PCR, reporter assays) to identify and validate miR-20a's target gene.

    Main Results:

    • MiR-20a expression is significantly elevated in bladder cancer tissues.
    • Higher miR-20a levels correlate with advanced histological grade, clinical stage, and metastasis.
    • Upregulation of miR-20a enhances bladder cancer cell proliferation, migration, and invasion in vitro.
    • LASS2 was identified and validated as a direct target gene negatively regulated by miR-20a.

    Conclusions:

    • Elevated miR-20a is associated with aggressive clinicopathological features in bladder cancer.
    • MiR-20a functions as an oncogene in bladder cancer by directly targeting LASS2.