Related Experiment Video
Updated: Oct 24, 2025

Overexpressing and Purifying a Toxic Nuclease from Escherichia coli
Published on: August 29, 2025
Xanthomonas oryzae pv. oryzae Exoribonuclease R Is Required for Complete Virulence in Rice, Optimal Motility, and
Sharmila Roy1, Pragya Mittal2, Lavanya Tayi3
1CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad, Telangana State, India 500007.
Abstract:
Exoribonuclease R (RNase R) is a 3' hydrolytic exoribonuclease that can degrade structured RNA. Mutation in RNase R affects virulence of certain human pathogenic bacteria. The aim of this study was to determine whether RNase R is necessary for virulence of the phytopathogen that causes bacterial blight in rice, Xanthomonas oryzae pv. oryzae (Xoo). In silico analysis has indicated that RNase R is highly conserved among various xanthomonads. Amino acid sequence alignment of Xoo RNase R with RNase R from various taxa indicated that Xoo RNase R clustered with RNase R of order Xanthomonadales. To study its role in virulence, we generated a gene disruption mutant of Xoo RNase R. The Xoo rnr mutant is moderately virulence deficient, and the complementing strain (rnr/pHM1::rnr) rescued the virulence deficiency of the mutant. We investigated swimming and swarming motilities in both nutrient-deficient minimal media and nutrient-optimal media. We observed that RNase R mutation has adversely affected the swimming and swarming motilities of Xoo in optimal media. However, in nutrient-deficient media only swimming motility was noticeably affected. Growth curves in optimal media at suboptimal temperature (15°C cold stress) indicate that the Xoo rnr mutant grows more slowly than the Xoo wild type and complementing strain (rnr/pHM1::rnr). Given these findings, we report for the first time that RNase R function is necessary for complete virulence of Xoo in rice. It is also important for motility of Xoo in media and for growth of Xoo at suboptimal temperature.
Related Concept Videos
Stringent Response in E. coli
Intracellular Movement of Viruses and Bacteria
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Other Stress Responses in Bacteria
Ribozymes
Ribozymes can...
Regulation of the Unfolded Protein Response

