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A novel biosensor based on multienzyme microcapsules constructed from covalent-organic framework.

Huihui Liang1, Linyu Wang1, Yuxi Yang1

  • 1College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, China.

Biosensors & Bioelectronics
|August 13, 2021
PubMed
Summary
This summary is machine-generated.

New enzyme microcapsules (enzymes@COF) improve electrochemical biosensors by protecting enzyme structure and activity. This approach offers enhanced performance for detecting glucose, H2O2, and malathion compared to traditional methods.

Keywords:
Covalent organic frameworkElectrochemical biosensorEnzymeMicrocapsule

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Area of Science:

  • * Electrochemistry
  • * Materials Science
  • * Biosensor Technology

Background:

  • * Enzyme immobilization on electrodes is crucial for biosensor development but often hinders enzyme activity due to conformational constraints.
  • * Existing methods using microporous frameworks (COF, MOF) show promise but may not fully ensure enzyme conformational expansion.
  • * Developing novel immobilization strategies is essential to enhance enzyme bioactivity and biosensor performance.

Purpose of the Study:

  • * To introduce a novel multienzyme microcapsule system (enzymes@COF) for constructing advanced electrochemical biosensors.
  • * To investigate the impact of a large cavity (600 nm) within the microcapsules on enzyme conformation and bioactivity.
  • * To evaluate the performance of the enzymes@COF microcapsule-based biosensor for detecting glucose, hydrogen peroxide (H2O2), and malathion.

Main Methods:

  • * Fabrication of multienzyme microcapsules (enzymes@COF) encapsulating glucose oxidase, horseradish peroxidase, and acetylcholinesterase within a 600 nm cavity and a COF shell.
  • * Characterization of the microcapsule structure, including cavity size and COF shell properties.
  • * Electrochemical detection of analytes (glucose, H2O2, malathion) using the fabricated enzymes@COF microcapsule-based biosensor.

Main Results:

  • * The 600 nm cavity within the enzymes@COF microcapsules allowed for free enzyme conformational expansion, preserving bioactivity.
  • * The COF shell provided chemical stability and protection against harsh environments, while allowing substrate diffusion.
  • * The biosensor demonstrated significantly improved performance, with low detection limits (e.g., 0.85 μM for glucose, 2.81 nM for H2O2, 3.0×10⁻¹³ g/L for malathion) and wide detection ranges.

Conclusions:

  • * Enzymes@COF microcapsules represent a feasible and effective strategy for fabricating high-performance electrochemical biosensors.
  • * The microcapsule design overcomes limitations of traditional enzyme immobilization, enhancing enzyme stability and activity.
  • * This approach holds significant potential for developing sensitive and reliable biosensing platforms for various analytes.