Chemotactic factors present in the supernatants of cultured sarcoid granulomas

The present study was undertaken to test whether cultured lymphocytes and epithelioid cells from sarcoid granulomas obtained from patients with active stage 2 sarcoidosis produced chemotactic factors (CF) for leukocytes as assessed by modified Boyden's method. The results indicate that: (1) CF for mononuclear cells and for neutrophils were detected in supernatants from cultured sarcoid granulomas; (2) similar activity was detected in culture supernatants from both lymphocytes and epithelioid cells; (3) CF present in the supernatants of cultured epithelioid cells were similar physically to those which other investigators have disclosed to be released by alveolar macrophages, (4) CF in the supernatants of cultured lymphocytes were similar to the lymphokines based on an estimation of molecular size; (5) neutrophils from patients with sarcoidosis were less responsive to zymosan-activated sera and did not respond well to CF derived from their own granuloma, whereas mononuclear cells from sarcoid patients responded well to CF. In conclusion, CF are present in the supernatants of cultured sarcoid granulomas and they appear to preferentially attract mononuclear cells from sarcoid patients.