Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

19.1K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
19.1K
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

6.6K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Iterative Bump-and-Hole Engineering Creates a Bioorthogonal Reporter for <i>N</i>-Acetylglucosaminyltransferase I.

Journal of the American Chemical Society·2026
Same author

Molecular Views into the Synthesis and Activation of Flavocytochrome <i>b</i><sub>558</sub> in Phagocytic Cells-Focus on the Role of EROS.

Antioxidants (Basel, Switzerland)·2026
Same author

Hemagglutinin double-mutation enhances binding of human-infecting avian influenza virus clade 2.3.4.4b H5Ny to human and SLe<sup>X</sup> receptors.

EMBO reports·2026
Same author

A long-chain heparan sulfate capture mechanism directs paracrine GDNF-GFRα1 signalling through RET.

bioRxiv : the preprint server for biology·2026
Same author

Structural Insights Into Man<sub>9</sub> Recognition by the HIV Antibody 2G12 Revealed by Paramagnetic NMR.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Enhancing lectin recognition via precise fluorination: Man<sub>5</sub> glycomimetics for targeting DC-SIGN.

Bioorganic chemistry·2026
Same journal

Azo compounds as key intermediates in the synthesis of cinnolines. Recent advances.

Organic & biomolecular chemistry·2026
Same journal

Cyclopentannulation toward hydrindane frameworks for the tricyclic core of (±)-rumphellclovane E and (±)-sarinfacetamides A and B.

Organic & biomolecular chemistry·2026
Same journal

Rosamine derivatives of <i>o</i>-aminothiophenol-triacetate (S-APTRA): a new class of selective fluorescent sensors for Zn<sup>2</sup>.

Organic & biomolecular chemistry·2026
Same journal

Sulfonamides: a decade of development in synthetic methods and biological activity studies.

Organic & biomolecular chemistry·2026
Same journal

TBAF-promoted dephenolative transcarbomylation: direct access to substituted carbamates & thiocarbamates.

Organic & biomolecular chemistry·2026
Same journal

Catalytic synthesis of saturated azacycles using transborylation.

Organic & biomolecular chemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 24, 2025

Author Spotlight: MAPP Protocol &#8211; Advancing Glycan Analysis
07:12

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

2.1K

Controlled density glycodendron microarrays for studying carbohydrate-lectin interactions.

Antonio Di Maio1, Anna Cioce, Silvia Achilli

  • 1Glycosystems Laboratory, Instituto de Investigaciones Químicas (IIQ), CSIC - Universidad de Sevilla, Av. Américo Vespucio 49, 41092 Seville, Spain. a.di-maio@imperial.ac.uk javier.rojo@iiq.csic.es.

Organic & Biomolecular Chemistry
|August 13, 2021
PubMed
Summary
This summary is machine-generated.

Researchers created glycodendron microarrays for studying lectin-carbohydrate interactions. Varying the density of these sugar-presenting molecules on a surface revealed how valency influences binding affinity.

More Related Videos

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.9K
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

7.0K

Related Experiment Videos

Last Updated: Oct 24, 2025

Author Spotlight: MAPP Protocol &#8211; Advancing Glycan Analysis
07:12

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis

Published on: September 29, 2023

2.1K
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

15.9K
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

7.0K

Area of Science:

  • Carbohydrate Chemistry
  • Biomolecular Interactions
  • Surface Science

Background:

  • Lectins are proteins that bind carbohydrates, playing roles in biological processes.
  • Understanding lectin-carbohydrate interactions is crucial for diagnostics and therapeutics.
  • Microarrays offer a platform for high-throughput screening of molecular interactions.

Purpose of the Study:

  • To construct glycodendron microarrays with controlled valency for lectin binding studies.
  • To investigate the influence of glycodendron surface density and valency on carbohydrate-lectin interactions.
  • To establish a method for in situ analysis of microarray synthesis and binding events.

Main Methods:

  • On-chip synthesis of glycodendrons on indium tin oxide (ITO) coated slides.
  • Spotwise strain-promoted azide-alkyne cycloaddition (SPAAC) for attaching glycans.
  • Non-covalent immobilization of dendrons via hydrophobic interactions.
  • In situ MALDI-TOF MS analysis for monitoring surface density and reaction conversion.

Main Results:

  • Successfully constructed glycodendron microarrays with defined valencies.
  • Demonstrated that carbohydrate-lectin interactions become exclusively valency-dependent at low surface densities.
  • Validated the use of MALDI-TOF MS for in situ analysis of microarray fabrication.
  • Studied interactions with various plant and human lectins.

Conclusions:

  • Glycodendron microarrays are effective tools for studying valency-dependent carbohydrate-lectin interactions.
  • Surface density is a critical factor modulating the observed binding affinity.
  • The developed on-chip synthesis and analysis method provides a robust platform for glycoarray development.