Related Experiment Video
Updated: Oct 24, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Methylated mud snail protein as a bio-flocculant for high turbidity wastewater treatment
Shaohui Mao1, Xiaohui Xu2, Linjiang Zhang1
1Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of the Ministry of Education, School of Water and Environment, Chang'an University, No. 126, Yanta Road, Xi'an 710054, Shaanxi, China.
Abstract:
The authors reported a potential candidate methylated mud snail protein (MeMsp) as an effective and eco-friendly flocculant to treat the high turbidity wastewater. MeMsp was obtained by extraction of mud snail protein (Msp) through isoelectric precipitation (PSC-IP) and then methylated via the esterification with side-chain carboxyl. Structural characterization of FT-IR, zeta potential and elemental analysis were carried out and further confirmed the successful of the methylation. Flocculation experiments with kaolin suspension simulated wastewater indicated that MeMsp-24 displayed more excellent flocculation efficiency at a low dosage. At the optimum dosage 27 mg/L, the maximum clarification efficiency of MeMsp-24 was 97.46% under pH 7.0. Furthermore, MeMsp-24 exhibited a wide flocculation window in the pH range 1.0-9.0, and faster sedimentation velocity and larger flocs size. In addition, MeMsp-24 exhibited 92.12% clarification efficiency in treating railway tunnel construction effluent. The flocculation kinetic and mechanism analysis revealed that the most effective particle collision occurred at the optimal dosage, with charge neutralization and adhesion playing irreplaceable roles in different environments, respectively. Therefore, through extraction and methylation modification, MeMsp could be a promising eco-friendly flocculant for high turbidity wastewater treatment.
Insights
Methylated mud snail protein (MeMsp) offers an eco-friendly solution for treating high turbidity wastewater. This modified protein demonstrates excellent flocculation efficiency and a wide pH operating range, making it a promising sustainable treatment option.
Area of Science:
- Environmental Science
- Biotechnology
- Materials Science
Background:
- High turbidity wastewater poses environmental challenges.
- Existing flocculants may have environmental drawbacks.
- Novel, eco-friendly flocculants are needed for effective wastewater treatment.
Purpose of the Study:
- To develop and evaluate methylated mud snail protein (MeMsp) as an eco-friendly flocculant.
- To investigate the structural characteristics and flocculation performance of MeMsp.
- To explore the application of MeMsp in treating high turbidity wastewater.
Main Methods:
- Mud snail protein (Msp) extracted via isoelectric precipitation (PSC-IP).
- Msp methylation achieved through esterification of side-chain carboxyl groups.
- Structural characterization using FT-IR, zeta potential, and elemental analysis.
- Flocculation experiments using kaolin suspension and railway tunnel construction effluent.
Main Results:
- Successful methylation confirmed by structural characterization.
- MeMsp-24 demonstrated high flocculation efficiency (97.46% clarification) at low dosage (27 mg/L) and neutral pH.
- MeMsp-24 exhibited a broad effective pH range (1.0-9.0), faster sedimentation, and larger flocs.
- Achieved 92.12% clarification efficiency on railway tunnel construction effluent.
- Flocculation mechanism involves charge neutralization and adhesion.
Conclusions:
- MeMsp is a highly effective and eco-friendly flocculant for high turbidity wastewater.
- Methylation significantly enhances the flocculation performance of mud snail protein.
- MeMsp shows potential for sustainable industrial wastewater treatment applications.
More Related Videos
10:53Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
Published on: March 17, 2023
10:37Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts
Published on: April 9, 2016