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The effectiveness of surface disinfectants and a micellic H2O2 based water disinfectant on Candida auris

Beata Zatorska1, Doris Moser2, Magda Diab-Elschahawi1

  • 1Department of Hospital Hygiene and Infection Control, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria.

Journal De Mycologie Medicale
|August 13, 2021
PubMed
Abstract

Insights

Ethanol and quaternary ammonium disinfectants effectively eliminate Candida biofilms. Potassium peroxymonosulfate also eradicated biofilms, though some cells remained viable, while hydrogen peroxide formulations showed rapid efficacy.

Area of Science:

  • Microbiology
  • Infection Control
  • Environmental Hygiene

Background:

  • Emerging multidrug-resistant strains of *Candida auris* pose significant challenges to infection control.
  • Hospital surface and water disinfectants require evaluation against *C. auris* biofilm forms.

Purpose of the Study:

  • To assess the efficacy of various hospital disinfectants against *Candida auris* biofilms.
  • To evaluate a novel water disinfectant formulation against *C. auris*.

Main Methods:

  • Tested *C. auris* and *C. albicans* strains against ethanol (ETH), quaternary ammonium (QAC), glutaraldehyde-based (ALD), and potassium peroxymonosulfate (PP) disinfectants.
  • Evaluated hydrogen peroxide (H2O2) and a micellar hydrogen peroxide formulation (mH2O2).
  • Measured disinfectant efficacy using tetrazolium salt reduction (XTT) assay and log10 reduction assay.

Main Results:

  • ETH and QAC achieved >5log10 reduction and >80% XTT reduction for all *Candida* biofilms and planktonic cells.
  • Potassium peroxymonosulfate (PP) eradicated all tested *C. auris* biofilms (>5log10 reduction), but XTT assay indicated persistent viable but non-culturable cells.
  • A 4.25% H2O2 formulation (25% mH2O2) demonstrated rapid killing of *Candida* biofilms within 1 minute.

Conclusions:

  • Ethanol and QAC-based disinfectants are highly effective against *C. auris* biofilms.
  • Potassium peroxymonosulfate shows broad-spectrum efficacy but requires further assessment of cell viability.
  • Hydrogen peroxide formulations offer rapid disinfection against *C. auris* biofilms.

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