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Updated: Oct 24, 2025

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Stimulated luminescence; Analysis of complex signals and fitting of dose response curves using analytical expressions
P Konstantinidis1, S Kioumourtzoglou1, G S Polymeris2
1Aristotle University of Thessaloniki, Physics Department, Nuclear Physics and Elementary Particles Physics Section, 54124, Thessaloniki, Greece.
Abstract:
Thermoluminescence (TL) and optically stimulated luminescence (CW-OSL, LM-OSL) are widely used by the scientific community in different applications. In order for the information of a glow or decay curve to be used correctly, the deconvolution of the corresponding curve is essential. Nowadays, there are plethora of deconvolution methods with none of them being dominant and universal. Recently, researchers created some new analytical equations for the TL, CW-OSL and the LM-OSL phenomena, based on the Lambert W function, with the use of a single master equation, as well as some new dose response curves based on a) the simple One Trap One Recombination (OTOR) model and b) the more complex Two Traps One Recombination (TTOR) model. The main aim of the study is to recreate these five expressions in different Excel spreadsheets, for a more practical use. The first step is the creation of a computational code of the Lambert W function in VBA at Excel. After that aim is achieved, five spreadsheets were created, namely the TL deconvolution, the CW-OSL deconvolution, the LM-OSL deconvolution, the OTOR model Dose response and the TTOR Dose response. In order to run some tests using these spreadsheets, four different sets of experimental data originated from different sources were used each time, with the calculated values being compared to the literature. In the end, the present study provides some notes and discussion about the applications of the newly created deconvolution spreadsheets.
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