Removal of difenoconazole and nitenpyram by composite calcium alginate beads during apple juice clarification

A novel genipin crosslinked calcium alginate/chitosan/polydopamine composite beads (g-Alg/CS/PDA) was synthesized for the removal of residual difenoconazole and nitenpyram during the clarification of apple juice. The composite beads with low potential health risks for all of the main materials were natural, green and biocompatible. Since g-Alg/CS/PDA can both clarify and adsorb, pesticide residues could be removed during the clarification of juice without additional steps. The g-Alg/CS/PDA beads were characterized, and the adsorption parameters, including the pesticide residue levels, adsorption time, pH, ionic strength, fructose concentration and adsorbent dose, were optimized. The adsorption data were fitted to the Freundlich isotherm model (R² = 0.9604, 0.9625) and the pseudo-second-order kinetic model (R² = 0.9993, 0.9999). The results indicated that the adsorption behavior of beads was heterogeneous. Moreover, the rate was controlled by several factors. The adsorption mechanism of two pesticides was also discussed. Hydrophobic and π-π conjugation interactions played a dominant role for the adsorption of difenoconazole, while hydrogen bonding and electrostatic interactions were the main factors for nitenpyram.