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Production of iota toxin by Clostridium spiroforme: a requirement for divalent cations
R J Carman1, R L van Tassell, T D Wilkins
1Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Veterinary Microbiology
|October 1, 1987
Abstract:
The effects of divalent cations (Ca2+, Co2+ and Zn2+) on the production of iota toxin by Clostridium spiroforme were studied. Toxin production had an absolute requirement for one or more cations in the range 1-5 mM. Using bispecific antisera, we showed that production of both the components of the toxin (ia and ib) were enhanced by divalent cations added to brain-heart infusion supplemented with peptone and glucose.