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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Published on: April 7, 2014

Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast.

Ranjan Kalita¹, William Flanagan¹, Jonathan Lightley¹

¹Photonics Group, Physics Department, Imperial College London, London, UK.

Journal of Biophotonics

|August 14, 2021

Summary

This summary is machine-generated.

We developed a low-cost differential phase microscopy method using a polarization-sensitive camera. This technique, polarization-resolved differential phase contrast (pDPC) microscopy, is easily integrated with fluorescence microscopy for advanced imaging.

Keywords:

phase contrast microscopy polarisation-resolved imaging quantitative phase imaging

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Phase Contrast and Differential Interference Contrast DIC Microscopy

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

Area of Science:

Microscopy
Optical Imaging
Biophysics

Background:

Differential phase contrast (DPC) microscopy visualizes transparent specimens without staining.
Traditional DPC methods often require complex setups or multiple acquisitions.
Integrating phase contrast with fluorescence microscopy is challenging.

Purpose of the Study:

To present a robust, low-cost, single-shot implementation of DPC microscopy.
To introduce polarization-resolved differential phase contrast (pDPC) microscopy.
To demonstrate the compatibility of pDPC with fluorescence microscopy.

Main Methods:

Utilized a polarization-sensitive camera to capture four simultaneous images.
Calculated phase contrast images from the acquired polarization-resolved data.
Integrated the pDPC technique with standard fluorescence microscopy setups.

Main Results:

Achieved a robust and low-cost single-shot DPC implementation.
Successfully computed phase contrast images from the four polarization-resolved acquisitions.
Demonstrated seamless integration of pDPC with fluorescence imaging.

Conclusions:

The developed pDPC microscopy offers a simple and cost-effective approach to phase contrast imaging.
This technique enhances existing fluorescence microscopes without significant modifications.
pDPC microscopy provides a valuable tool for biological and materials science research.