Related Experiment Video

Updated: Oct 24, 2025

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR
08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.7K

A circular RNA, circSMARCA5, inhibits prostate cancer proliferative, migrative, and invasive capabilities via the

Xin Xie1, Fu-Kang Sun1, Xin Huang1

  • 1Department of Urology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.

Aging
|August 14, 2021
PubMed

Insights

Circular RNA SMARCA5 (circSMARCA5) is downregulated in prostate cancer, suppressing metastasis and growth. It regulates TIMP3 expression via miR-181b-5p/miR-17-3p, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The role of SMARCA5 (circSMARCA5) in prostate cancer progression and metastasis is not well understood.
  • circSMARCA5 is implicated in various cancers, but its specific function in prostate cancer remains elusive.

Purpose of the Study:

  • To investigate the functional significance of circSMARCA5 in prostate cancer.
  • To elucidate the molecular mechanisms underlying circSMARCA5's role in prostate cancer carcinogenesis and metastasis.

Main Methods:

  • Quantitative real-time PCR and Western blot assays were used to assess circSMARCA5 and TIMP3 expression.
  • RNA immunoprecipitation, biotin-coupled microRNA capture, and luciferase reporter assays were employed to determine molecular interactions.
  • Prostate cancer tissues and cell lines were utilized for functional and mechanistic studies.

Main Results:

  • circSMARCA5 expression was significantly downregulated in primary and metastatic prostate cancer tissues compared to normal controls.
  • circSMARCA5 demonstrated a suppressive effect on prostate cancer cell growth and metastasis.
  • circSMARCA5 regulates Tissue Inhibitor of Metalloproteinases 3 (TIMP3) expression through interactions with miR-181b-5p and miR-17-3p.
  • Lysine acetyltransferase 5 (KAT5) was found to induce circSMARCA5 biogenesis and modulate the miR-181b-5p-TIMP3/miR-17-3p-TIMP3 axis.

Conclusions:

  • circSMARCA5 acts as a tumor suppressor in prostate cancer by inhibiting metastasis and growth.
  • The circSMARCA5-miR-181b-5p-TIMP3 and circSMARCA5-miR-17-3p-TIMP3 axes represent novel molecular pathways in prostate cancer.
  • Targeting these circSMARCA5-mediated pathways could offer a new therapeutic strategy for prostate cancer treatment.
Keywords:
TIMP3circSMARCA5miR-17-3pmiR-181b-5pprostate cancer

More Related Videos

miRNA Expression Analyses in Prostate Cancer Clinical Tissues11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

11.0K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.6K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.6K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.1K