Preparation and characterization of decellularized rooster comb as a scaffold for tissue engineering applications

Decellularization is a method that has been widely used in tissue engineering especially in the last 20 years. In this study decellularized rooster comb was prepared and characterized for using it as a tissue scaffold. Treatment of tissues with sodium dodecyl sulfate (SDS) and Triton X-100 as two decellularization procedures in different time points were compared according to different parameters such as cytocompatibility, cell removal, preservation of extracellular matrix (ECM), and mechanical properties to find the optimum technique. Even though there was no difference regarding to efficiency on cell removal, SDS demonstrated better results on protection of tissue morphology in comparison with Triton X-100. Therefore, in general the samples treated with SDS showed higher levels of mechanical properties and cytocompatibility in comparison with Triton X-100 applied tissues. In the cuisines of many countries, rooster comb is discarded as a waste material however, in this study it was demonstrated that decellularized rooster comb could be utilized as a cheap, easily obtainable, and biocompatible scaffold. In conclusion, it was revealed that decellularized rooster comb is a promising biomaterial for using as scaffold and it is expected to be utilized for the further studies in particular on skin tissue engineering.