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Updated: Aug 11, 2026
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Published on: December 25, 2016
Quantitative image analysis method for detection of nitrite with cyanine dye-NaYF4:Yb,Tm@NaYF4 upconversion
Hongqi Chen1, Wei Tang1, Yunchun Liu1
1Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Abstract:
In this work, a quantitative image analysis method based on cyanine dye-upconversion nanoparticles composite luminescent nanoprobe for the detection of nitrite was developed. The nanoprobe was constructed by combining the NaYF4:Yb,Tm@NaYF4 upconversion nanoparticles (UCNPs) and the new cyanine dye IR-790. The upconversion nanoparticles transferred energy to IR-790, resulting in the luminescence quenching, while the luminescence of UCNPs was recovered after adding NO2-. The increase in photons was related to the concentration of NO2-. Under the optimal experimental conditions, the detection range was 0.20-140 μM and the limit of detection was 0.030 μM. The measurement for NO2- can be completed in 29 min. The method has the characteristics of fast response (~0.1 s), low sample consumption (10 μL) and powerful data support (550 frame time series images). Furthermore, the quantitative image analysis method was successfully applied for the analysis of nitrite in environmental water and food samples.
