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Updated: Oct 1, 2026
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
Preparation of leukocyte-poor platelet concentrates from buffy coats. I. Special inserts for centrifuge cups
R N Pietersz1, H W Reesink, W J Dekker
1Red Cross Blood Bank, Amsterdam, The Netherlands.
A special insert was developed for centrifuge cups in order to prepare leukocyte-poor platelet concentrates from buffy coats by using quadruple citrate phosphate dextrose-saline adenine glucose mannitol systems from different manufacturers. Each centrifuge cup could contain up to 4 sets of double bags allowing the preparation of 24 platelet concentrates per run. Optimal conditions for centrifugation of the buffy coats in the inserts were found to be 6 min at 380 g (2,150 g min). A platelet count of 69 +/- 19 X 10(9) and a leukocyte contamination of 14 +/- 10.5 X 10(6) per platelet concentrate was thereby obtained in a plasma volume of 63 +/- 10.5 ml (mean +/- SD). The method described allows large scale production of leukocyte-poor platelet concentrates from buffy coats in a closed system.
A special insert was developed for centrifuge cups in order to prepare leukocyte-poor platelet concentrates from buffy coats by using quadruple citrate phosphate dextrose-saline adenine glucose mannitol systems from different manufacturers. Each centrifuge cup could contain up to 4 sets of double bags allowing the preparation of 24 platelet concentrates per run. Optimal conditions for centrifugation of the buffy coats in the inserts were found to be 6 min at 380 g (2,150 g min). A platelet count of 69 +/- 19 X 10(9) and a leukocyte contamination of 14 +/- 10.5 X 10(6) per platelet concentrate was thereby obtained in a plasma volume of 63 +/- 10.5 ml (mean +/- SD). The method described allows large scale production of leukocyte-poor platelet concentrates from buffy coats in a closed system.
