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Related Concept Videos

Clathrin Coated Vesicles01:12

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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Cell Migration01:19

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Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
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COP Coated Vesicles00:59

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Related Experiment Video

Updated: Oct 24, 2025

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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Migration cues interpretation by clathrin-coated structures.

Nadia Elkhatib1, Kesniia Porshneva1, Guillaume Montagnac1

  • 1Inserm U1279, Gustave Roussy Institute, Université Paris-Saclay, Villejuif, France.

Current Opinion in Cell Biology
|August 14, 2021
PubMed
Summary

Cell surface receptors cluster in clathrin-coated structures to guide cell migration direction. This clustering regulates receptor availability and integrates environmental cues for directed cell movement.

Keywords:
AdhesionCell migrationClathrin-mediated endocytosisExtracellular cuesSignalingTrafficking

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Area of Science:

  • Cell Biology
  • Biophysics
  • Molecular Biology

Background:

  • Cell migration relies on interpreting environmental cues.
  • Cell surface receptors detect external signals and are crucial for migration direction.
  • Receptor internalization and clustering influence cell migration decisions.

Purpose of the Study:

  • To investigate the role of receptor clustering in plasma membrane domains.
  • To understand how clathrin-coated structures regulate receptor availability and cell migration.
  • To explore the involvement of clathrin-coated structures in feedback mechanisms for migration direction.

Main Methods:

  • Analysis of receptor dynamics at the plasma membrane.
  • Investigating the formation and regulation of clathrin-coated structures.
  • Studying the impact of receptor clustering on cell migration phenotypes.

Main Results:

  • Receptor clustering at discrete plasma membrane locations facilitates signal interpretation.
  • Clathrin-coated structures act as key assemblies for controlling receptor availability.
  • These structures are regulated by factors influencing cell migration, forming feedback loops.

Conclusions:

  • Clathrin-coated structures are essential for translating environmental cues into directed cell migration.
  • Receptor clustering, independent of endocytosis, is a critical mechanism for migration steering.
  • Clathrin-coated structures play a significant role in feedback loops that define cell migration direction.