An online, non-destructive method for simultaneously detecting chemical, biological, and physical properties of...

Botanical drugs hold great potential to prevent and treat complex diseases. Quality control is essential in ensuring the safety, efficacy, and therapeutic consistency of these drug products. The quality of a botanical drug product can be assessed using a variety of analytical methods based on criteria that judge the identity, strength, purity, and potency. However, most of these methods are developed on separate analytical platforms, and few approaches are available for in-process monitoring of multiple quality properties in a non-destructive manner. Here, we present a hyperspectral imaging-based strategy for online measurement of physical, chemical, and biological properties of botanical drugs using artificial intelligence algorithms. An end-to-end convolutional neural network (CNN) model was established to accurately determine phytochemicals and bioactivities based on the spectra. Besides, a new dual-scale anomaly (DSA) detection algorithm was proposed for visible particle inspection based on the images. The strategy was exemplified on Shuxuening Injection, a Ginkgo biloba-derived drug used in the treatment of cerebrovascular and cardiovascular diseases. Four quality metrics of the injection, including total flavonol, total ginkgolides, antioxidant activity, and anticoagulant activity, were successfully predicted by the CNN model with validation R² of 0.922, 0.921, 0.880, and 0.913 respectively, showing better performance than the other models. Unqualified samples with visible particles could be detected by DSA with a low false alarm rate of 9.38 %. Chromaticity results indicated that the inter-company variations of color were significant, while intra-company variations were relatively small. This demonstrates a real application of integrating hyperspectral imaging with artificial intelligence to provide a rapid, accurate, and non-destructive approach for process analysis of botanical drugs.