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Related Experiment Videos

Active chromatin structure.

D M Lilley

    Cell Biology International Reports
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Active genes reside in nucleosomes, with histones attached to transcribing DNA. Active nucleosomes exhibit altered conformations, making DNA more susceptible to DNase I, indicating potential transcriptional activity.

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    Area of Science:

    • Molecular Biology
    • Chromatin Biology
    • Gene Expression

    Background:

    • Active gene sequences are found within the nucleosomal fraction of chromatin.
    • Histones remain associated with the DNA of transcribing non-ribosomal genes.
    • Electron microscopy suggests histone conformation relates to nucleosome structure.

    Purpose of the Study:

    • To investigate the conformational changes in nucleosomes associated with active gene sequences.
    • To understand the relationship between nucleosome structure and transcriptional activity.
    • To explore proposed mechanisms for nucleosome disassembly during transcription.

    Main Methods:

    • Analysis of the nucleosomal fraction of chromatin.
    • Electron microscopy (EM) imaging of histone-DNA complexes.

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  • Assessment of DNA susceptibility to endonucleolytic attack by DNase I.

    • Main Results:

    • Active gene sequences are located in the nucleosomal fraction.
    • Histones are attached to DNA in transcribing genes, with conformations resembling nucleosomes.
    • Active nucleosomes display altered conformations, evidenced by increased DNA susceptibility to DNase I.
    • This DNA alteration suggests potential rather than ongoing transcriptional activity.

    Conclusions:

    • Nucleosomes undergo conformational changes that correlate with potential gene activity.
    • Proposed mechanisms for transcription involve nucleosome disassembly via altered histone-histone interactions, preserving histone-DNA contact.