Related Experiment Video
Updated: Oct 24, 2025
Chromatin Immunoprecipitation in the Cnidarian Model System Exaiptasia diaphana
Published on: March 17, 2023
Insights from the cDNA and EST analysis of Antrodia cinnamomea
Jing Li1, Yanling Liu1, Richard Yankey1,2
1National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and orestry University, Fujian, Fuzhou 350002, PR China.
Abstract:
It is of interest to document the insights gleaned from the cDNA and EST analysis of Antrodia cinnamomea (a fungal species). Hence a library of sequences was constructed and analysed using standard procedures to gain new insights. Therefore, 65 ESTs, with size ranging from 300-2000 bp, were constructed. This included 46 ESTs with definite annotation, 18 ESTs were hypothetical and 1 new protein derived from BLAST analysis. We assigned 227 Gene Ontology terms linked to cell composition, transport, catalytic activity, and regulation functions in these sequences. Moreover, 56 matching genes were found in 8 Kyoto Encyclopedia of Genes and Genomes pathways. Data also showed 271 SSRs from Antrodia cinnamomea ESTs with an occurrence frequency of 96.82%. The STRING data analysis showed 29 genes encoded enzymes highly involved in protein-to-protein interactions linked to expression of regulation function. Thus, we documented some insights from the cDNA and EST analysis of Antrodia cinnamomea for further data mining.
