In vitro Nitrate Reductase Activity Assay of Mycolicibacterium smegmatis Crude Extract
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Published on: December 14, 2016
Wei Tan1,2, Zhi-Hui Shao3, Guo-Ping Zhao1,2,3
1Department of Microbiology, The Chinese University of Hong Kong, Hong Kong, China.
Abstract:
Nitrate is one of the major inorganic nitrogen sources for microorganisms. Many bacterial and archaeal lineages can express cytoplasmic assimilatory nitrate reductase (NAS), which catalyzes the rate-limiting reduction of nitrate to nitrite in the nitrate assimilation pathway. Here, we present a detailed protocol for measuring in vitro nitrate reductase (NaR) activity of NAS enzymes from Mycolicibacterium smegmatis crude extract using both physiological and non-physiological electron donors.
