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FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans
1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Bio-Protocol
|August 16, 2021
Abstract:
FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes in certain mutants.

