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Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry
Bingbing Ren1, Ahmed Mahmoud Mohammed Sayed2, Hwei Ling Tan1,3
1Department of Biochemistry, National University of Singapore, Yong Loo Lin School of Medicine, Singapore.
Bio-Protocol
|August 16, 2021
Abstract:
Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.
Keywords:
Bio-layer interferometryBiosensorHistone post-translational modificationLabel freePeptideProtein interactionRecombinant protein
