Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation02:17

Reduction of Alkenes: Asymmetric Catalytic Hydrogenation

3.6K
Catalytic hydrogenation of alkenes is a transition-metal catalyzed reduction of the double bond using molecular hydrogen to give alkanes. The mode of hydrogen addition follows syn stereochemistry.
The metal catalyst used can be either heterogeneous or homogeneous. When hydrogenation of an alkene generates a chiral center, a pair of enantiomeric products is expected to form. However, an enantiomeric excess of one of the products can be facilitated using an enantioselective reaction or an...
3.6K
Properties of Organometallic Compounds01:23

Properties of Organometallic Compounds

1.2K
Organometallic compounds are compounds that contain a carbon–metal bond. Carbon belongs to an organyl group like alkyl, aryl, allyl, or benzyl groups. The metal can be from Group I or Group II of the periodic table, a transition metal, or a semimetal.
1.2K
Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation02:24

Reduction of Alkynes to cis-Alkenes: Catalytic Hydrogenation

8.3K
Introduction
Like alkenes, alkynes can be reduced to alkanes in the presence of transition metal catalysts such as Pt, Pd, or Ni. The reaction involves two sequential syn additions of hydrogen via a cis-alkene intermediate.
8.3K
Synthesis of α-Substituted Carbonyl Compounds: The Stork Enamine Reaction01:26

Synthesis of α-Substituted Carbonyl Compounds: The Stork Enamine Reaction

3.6K
α-Substituted ketones or aldehydes can be synthesized from enamines by the Stork enamine reaction, named after its pioneer Gilbert Stork. Enamines are useful synthetic intermediates where the lone pair on nitrogen is in conjugation with the C=C bond. They resemble enolate ions, as the resonance forms of both species have a nucleophilic α carbon.
3.6K
Reduction of Alkenes: Catalytic Hydrogenation02:13

Reduction of Alkenes: Catalytic Hydrogenation

12.8K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
12.8K
Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)00:53

Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)

2.0K
Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Turning Motion into Methane: Electromagnetic Induction in Microbial Aggregates Enhances Wastewater Resource Recovery.

Environmental science & technology·2026
Same author

Nanozyme-engineered liners for proactive prevention of wear particle-induced osteolysis.

Nature communications·2026
Same author

Enzyme-Catalyzed Stereoselective C(sp<sup>3</sup>)-S Bond Formation via a Dichotomic Carbene Transfer Mechanism.

Journal of the American Chemical Society·2026
Same author

Spatial management of riverine methane (CH<sub>4</sub>) emissions based on landscape drivers in the Yangtze River Basin.

Journal of environmental management·2026
Same author

Highly stereoselective synthesis of allylic β-lactams <i>via</i> enzymatic C(sp<sup>3</sup>)-H amidation.

Chemical science·2026
Same author

Ecologically Informed Design of Synthetic Microbial Community Enables Robust Degradation and Engraftment for Antibiotic Removal in Wastewater.

Environmental science & technology·2026

Related Experiment Video

Updated: Oct 24, 2025

Facile Preparation of 2Z,4E-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate
06:46

Facile Preparation of 2Z,4E-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate

Published on: June 21, 2017

7.6K

Engineered and Artificial Metalloenzymes for Selective C-H Functionalization.

Xinkun Ren1, Rudi Fasan1

  • 1Department of Chemistry, University of Rochester, Hutchison Hall, 120 Trustee Rd, Rochester NY 14627, USA.

Current Opinion in Green and Sustainable Chemistry
|August 16, 2021
PubMed
Summary

Engineered metalloenzymes offer precise control over C-H bond functionalization for complex organic synthesis. This review details biocatalytic advances in selective oxyfunctionalization, halogenation, amination, and carbene insertion reactions.

More Related Videos

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs
08:25

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs

Published on: January 17, 2020

7.5K
A Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis
07:06

A Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis

Published on: February 16, 2020

8.3K

Related Experiment Videos

Last Updated: Oct 24, 2025

Facile Preparation of 2Z,4E-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate
06:46

Facile Preparation of 2Z,4E-Dienamides by the Olefination of Electron-deficient Alkenes with Allyl Acetate

Published on: June 21, 2017

7.6K
Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs
08:25

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs

Published on: January 17, 2020

7.5K
A Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis
07:06

A Microwave-Assisted Direct Heteroarylation of Ketones Using Transition Metal Catalysis

Published on: February 16, 2020

8.3K

Area of Science:

  • Organic Chemistry
  • Biocatalysis
  • Enzyme Engineering

Background:

  • Direct C-H bond functionalization is crucial for organic synthesis but faces challenges in selectivity for complex molecules.
  • Metalloenzymes provide a tunable platform for catalyst-controlled C-H bond functionalization through protein engineering and cofactor modification.

Purpose of the Study:

  • To review recent advancements in engineered and artificial metalloenzymes for C-H functionalization.
  • To highlight biocatalytic strategies for selective C-H oxyfunctionalization, halogenation, amination, and carbene insertion.

Main Methods:

  • Focus on engineered heme- and non-heme iron-dependent enzymes.
  • Exploration of abiological nitrene and carbene transfer chemistries.
  • Protein engineering and cofactor redesign for tuning enzyme reactivity and selectivity.

Main Results:

  • Engineered metalloenzymes demonstrate high chemo-, regio-, and stereocontrol in C-H functionalization.
  • Tunable selectivity achieved through enzyme design and cofactor environment.
  • Successful application in selective C-H oxyfunctionalization, halogenation, amination, and carbene insertion.

Conclusions:

  • Engineered metalloenzymes represent a powerful and sustainable approach to organic synthesis.
  • These biocatalytic systems expand synthetic toolboxes for generating chiral building blocks and late-stage functionalization.
  • Opens new avenues for natural product total synthesis and complex molecule diversification.