Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASD mutations in the ciliary gene CEP41 impact development of projection neurons and interneurons in a human cortical organoid model.

Molecular psychiatry·2026
Same author

Astrocyte Proximity Protects Synapses From Human Amyloid-Beta Induced Degeneration in a Mouse Ex Vivo Model of Early Alzheimer's Disease.

The European journal of neuroscience·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Longitudinal [<sup>18</sup>F]LW223 PET imaging of macrophage-driven inflammation following myocardial infarction in a rat model: implications for left ventricular remodelling.

European journal of nuclear medicine and molecular imaging·2025
Same author

Molecular profiling of brain endothelial cell to astrocyte endfoot communication in mouse and human.

Nature communications·2025
Same author

Prenatal SMN-dependent defects in translation uncover reversible primary cilia phenotypes in spinal muscular atrophy.

JCI insight·2025

Related Experiment Video

Updated: Oct 24, 2025

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
11:37

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Published on: August 2, 2017

10.0K

Microarray profiling emphasizes transcriptomic differences between hippocampal in vivo tissue and in vitro cultures.

Declan King1, Paul A Skehel2, Owen Dando2

  • 1Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, Edinburgh EH8 9JZ, UK.

Brain Communications
|August 16, 2021
PubMed
Summary

Primary hippocampal cell cultures offer a valuable in vitro model for brain research. Transcriptomic profiling reveals gene expression is largely conserved between these cultures and intact brain tissue, even in disease models.

Keywords:
hippocampal culturesmicroarrayneuronaltranscriptome

More Related Videos

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
08:32

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

Published on: October 25, 2016

13.6K
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
09:17

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Published on: March 4, 2015

9.7K

Related Experiment Videos

Last Updated: Oct 24, 2025

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit
11:37

Tuning in the Hippocampal Theta Band In Vitro: Methodologies for Recording from the Isolated Rodent Septohippocampal Circuit

Published on: August 2, 2017

10.0K
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
08:32

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

Published on: October 25, 2016

13.6K
Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue
09:17

Ex Utero Electroporation and Organotypic Slice Culture of Mouse Hippocampal Tissue

Published on: March 4, 2015

9.7K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Primary hippocampal cell cultures are widely used in vitro models for studying brain tissue.
  • The extent to which these cultures recapitulate the in vivo environment remains an important question.

Purpose of the Study:

  • To compare transcriptomic profiles of primary hippocampal cell cultures with intact hippocampal tissue.
  • To assess gene conservation across different genetic backgrounds, including a prion disease model.

Main Methods:

  • Transcriptomic profiling (RNA sequencing) of primary hippocampal cell cultures and intact hippocampal tissue.
  • Comparison of gene expression patterns between wild-type and PrP 101LL transgenic mice.

Main Results:

  • Significant conservation of gene expression profiles between primary hippocampal cultures and in vivo hippocampal tissue.
  • Gene expression patterns are largely conserved across genetically altered lines, including the prion disease model.

Conclusions:

  • Primary hippocampal cell cultures serve as a representative model for in vivo hippocampal tissue at the transcriptomic level.
  • The findings support the use of these cultures for studying gene expression in neurological contexts, including prion disease.