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Related Concept Videos

Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

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Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
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Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
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Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

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Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Ambient electric arc ionization for versatile sample analysis using mass spectrometry.

Yuanji Gao1,2, Yuan Li1, Binpeng Zhan1

  • 1Department of Chemistry, Zhejiang University, Hangzhou 310027, Zhejiang, P. R. China. panyuanjiang@zju.edu.cn.

The Analyst
|August 16, 2021
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Summary
This summary is machine-generated.

A new ambient electric arc ionization (AEAI) device offers versatile mass spectrometry analysis for diverse organic compounds. This soft ionization technique shows potential as an alternative to traditional methods like electrospray ionization.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Traditional ionization techniques in mass spectrometry have limitations.
  • A need exists for versatile and convenient ion sources.

Purpose of the Study:

  • To develop and characterize a novel ambient electric arc ionization (AEAI) device.
  • To evaluate AEAI as a soft ionization source for mass spectrometry.

Main Methods:

  • Development of a new ambient electric arc ionization (AEAI) device.
  • Coupling AEAI with a high-resolution Orbitrap mass spectrometer.
  • Analysis of various organic compounds with a wide range of polarities.

Main Results:

  • AEAI functions as a soft ionization technique, producing primarily protonated ions ([M + H]+).
  • Minimal in-source fragmentation was observed for most analytes.
  • Successful analysis of non-polar polybenzenoid aromatic hydrocarbons (PAHs) to highly polar amino acids.
  • Demonstrated versatility across a broad polarity spectrum.

Conclusions:

  • AEAI is a versatile and convenient ion source for mass spectrometry.
  • AEAI shows potential as an alternative to established ionization methods (ESI, APCI, EI).
  • The study discusses the limitations of the AEAI technique.