Related Experiment Video

Updated: Oct 24, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.3K

Exploring diagnostic and prognostic predictive values of microRNAs for acute myocardial infarction: A

Gien-Kuo Lee1,2,3, Yen-Ping Hsieh4, Shang-Wei Hsu1

  • 1Department of Healthcare Administration, Asia University, Taichung, Taiwan.

Medicine
|August 16, 2021
PubMed
Abstract

Insights

MicroRNAs (miRNAs) show diagnostic potential for acute myocardial infarction (AMI). Specific miRNAs like miR-1, miR-133, miR-208, and miR-499 demonstrate significant accuracy in diagnosing AMI, with miR-208 also linked to post-AMI mortality.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiology

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs with regulatory roles.
  • Previous studies on miRNA diagnostic and prognostic values for acute myocardial infarction (AMI) have yielded inconsistent results.
  • Biomarkers for early and accurate diagnosis of AMI are crucial for timely intervention and improved patient outcomes.

Purpose of the Study:

  • To systematically evaluate the diagnostic and prognostic predictive values of specific microRNAs for acute myocardial infarction (AMI).
  • To consolidate existing evidence through meta-analysis to provide a clearer understanding of miRNA utility in AMI.
  • To identify reliable miRNA biomarkers for AMI diagnosis and prognosis.

Main Methods:

  • Systematic literature search on PubMed and Web of Science for studies on miRNAs and AMI (1989-2019).
  • Meta-analysis of diagnostic accuracy studies, calculating sensitivity, specificity, PLR, NLR, and DOR.
  • Construction of summary receiver-operating characteristic (SROC) curves.
  • Meta-analysis of follow-up studies to compute hazard ratios (HRs) and confidence intervals (CIs) for prognostic values.

Main Results:

  • miR-1 demonstrated a sensitivity of 0.72 and specificity of 0.88 for AMI diagnosis.
  • miR-133 showed a sensitivity of 0.73 and specificity of 0.88 for AMI.
  • miR-208 exhibited high diagnostic accuracy with sensitivity of 0.83 and specificity of 0.96 for AMI.
  • miR-208 was significantly associated with increased mortality after AMI (HR 1.09).
  • miR-499 presented a sensitivity of 0.84 and specificity of 0.97 for AMI diagnosis.

Conclusions:

  • Circulating miR-1, miR-133, miR-208, and miR-499 possess significant diagnostic value for acute myocardial infarction (AMI).
  • miR-208 also holds prognostic significance, indicating a potential role in predicting mortality post-AMI.
  • These miRNAs represent promising biomarkers for the clinical management of AMI.

More Related Videos

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

12.1K
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening08:14

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

15.8K

Related Concept Videos

Acute Coronary Syndrome III: Diagnostic Studies01:30

Acute Coronary Syndrome III: Diagnostic Studies

Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
49
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.5K