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Updated: Oct 24, 2025

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Published on: February 27, 2020
Cryo-EM targets in CASP14
Tristan Cragnolini1, Andriy Kryshtafovych2, Maya Topf3
1Institute of Structural and Molecular Biology, Birkbeck, University College London, London, UK.
None:
Structures of seven CASP14 targets were determined using cryo-electron microscopy (cryo-EM) technique with resolution between 2.1 and 3.8 Å. We provide an evaluation of the submitted models versus the experimental data (cryo-EM density maps) and experimental reference structures built into the maps. The accuracy of models is measured in terms of coordinate-to-density and coordinate-to-coordinate fit. A-posteriori refinement of the most accurate models in their corresponding cryo-EM density resulted in structures that are close to the reference structure, including some regions with better fit to the density. Regions that were found to be less "refineable" correlate well with regions of high diversity between the CASP models and low goodness-of-fit to density in the reference structure.
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Cryo-electron Microscopy
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