IL-10 Receptor Neutralization-Induced Colitis in Mice: A Comprehensive Guide

Area of Science:

• Immunology
• Gastroenterology
• Animal Models

Background:

• Interleukin-10 (IL-10) and its receptor (IL-10R) are key in inflammatory bowel disease (IBD) pathogenesis.
• IL-10-deficient mice have limitations for studying immune-mediated colitis and testing therapies.
• Delayed and variable disease onset in current models hinders therapeutic development.

Purpose of the Study:

• To provide guidance on using anti-IL-10R monoclonal antibody (αIL-10R mAb) neutralization as a superior model for IBD research.
• To establish a reliable method for inducing chronic colitis in mice.
• To facilitate mechanistic studies and therapeutic efficacy assessments for IBD.

Main Methods:

• Induction of chronic colitis using αIL-10R mAb neutralization in mice.
• Biochemical evaluation of induced chronic colitis.
• Development of in-house assays for assessing colitis and inflammatory parameters.
• Protocols for stool analysis, scoring, and the Swiss roll method.

Main Results:

• αIL-10R mAb neutralization reliably induces chronic colitis within 4 weeks.
• This model allows for time-dependent mechanistic studies.
• The model is suitable for assessing the efficacy of IBD therapeutics.

Conclusions:

• αIL-10R mAb neutralization is a superior alternative to IL-10-deficient mice for IBD research.
• This model closely mimics immune-mediated colitis relevant to human Crohn's disease.
• The described protocols enable robust assessment of colitis and therapeutic responses.