The impact of silver sulfide nanoparticles and silver ions in soil microbiome

The use of biosolids as fertilizers in agriculture can lead to the exposure of soil biota to sulfidised silver nanoparticles (Ag₂S NPs), generated during the wastewater treatment procedures. Considering the crucial role of microorganisms on soil functions, we aimed to study the effects of 10 mg kg^-1 soil of Ag₂S NPs or AgNO₃ on the soil microbiome, using an indoor mesocosm. After 28 days of exposure, Ag₂S NPs induced a significant change in the soil microbiome structure, at class, genera and OTU levels. For instance, a significantly higher abundance of Chitinophagia, known for its lignocellulose-degrading activity, was observed in Ag₂S NPs-treated soil toward the control. Nevertheless, stronger effects were observed in AgNO₃-treated soil, over time, due to its higher silver dissolution rate in porewater. Additionally, only the AgNO₃-treated soil stimulates the abundance of ammonia-oxidizing (AOB; amoA gene) and nitrite-oxidizing (NOB; nxrB gene) bacteria, which are involved in the nitrification process. Distinct variants of amoA and nxrB genes emerged in silver-treated soils, suggesting a potential succession of AOB and NOB with different degree of silver-tolerance. Our study highlights the latter effects of Ag₂S NPs on the soil microbiome composition, while AgNO₃ exerted a stronger effect in both composition and functional parameters.