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Adherent cell thawing by infrared radiation.

Akalabya Bissoyi1, Ido Braslavsky1

  • 1The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition, The Hebrew University of Jerusalem, Rehovot, 7610001, Israel.

Cryobiology
|August 17, 2021
PubMed
Summary

Infrared radiation enables rapid thawing of cryopreserved adherent cells, improving viability and attachment. This fast thawing technique offers a significant advantage for cell therapy and tissue engineering applications.

Keywords:
Cell–on–chipCryoprotectant toxicityFast thawingInfrared thawingOrganoid cryopreservation

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Cryobiology

Background:

  • Cryopreservation of adherent cells is vital for cell therapy, but thawing methods can impact cell viability and attachment.
  • Current thawing techniques, like water bath thawing, are slow and can cause cell detachment.
  • Fast thawing methods using radiofrequency or laser heating have shown promise but have limitations.

Purpose of the Study:

  • To investigate the efficacy of infrared radiation for rapid thawing of cryopreserved adherent cells.
  • To compare infrared thawing with conventional water bath thawing in terms of cell viability and adhesion.
  • To explore the potential of infrared thawing for applications in cell therapy and tissue engineering.

Main Methods:

  • Utilized a focused halogen illuminator to deliver intense infrared radiation for rapid sample thawing.
  • Employed slow freezing for cryopreservation of two epithelial cell lines: retinal pigment epithelium and colorectal adenocarcinoma cells.
  • Assessed post-thawing cell viability and adhesion rates, comparing infrared thawing with water bath thawing.

Main Results:

  • Achieved rapid thawing of 2-4 mm thick samples within seconds, with heating rates in the thousands of Kelvin per minute.
  • Observed significantly higher post-thawing cell viability and cell adhesion rates with infrared thawing compared to water bath thawing.
  • Demonstrated effective cryopreservation and recovery of adherent epithelial cell lines using slow freezing and fast infrared thawing.

Conclusions:

  • Intense infrared radiation provides a highly effective and rapid method for thawing cryopreserved adherent cells.
  • Infrared thawing offers advantages over surface warming methods, potentially reducing cell stress and improving outcomes for adherent cells.
  • This technology holds promise for advancing cell therapy, tissue engineering, 3D scaffolds, organoid preservation, and lab-on-chip systems.