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Optimization of artificial urine formula for in vitro cellular study compared with native urine
Kanyarat Sueksakit1, Visith Thongboonkerd1
1Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
International Journal of Medical Sciences
|August 17, 2021
Summary
Adding fetal bovine serum (FBS) to artificial urine (AU) improved renal cell health. The optimized AU-Siriraj+2.5% FBS formula enhanced cell function and polarization for in vitro studies.
Area of Science:
- Nephrology
- Cell Biology
- Biochemistry
Background:
- Artificial urine (AU) formulations often lack proteins found in native urine.
- Physiological protein levels in urine may be crucial for renal cell health and function.
Purpose of the Study:
- To investigate the impact of fetal bovine serum (FBS) supplementation in artificial urine on renal tubular cells.
- To determine optimal conditions for in vitro studies using artificial urine.
Main Methods:
- MDCK renal tubular cells were cultured using the AU-Siriraj protocol with varying FBS concentrations (0-10%).
- Evaluated cell viability, time to deformation (TD), and transepithelial electrical resistance (TER).
Main Results:
- AU-Siriraj+2.5% FBS showed the longest TD compared to other AU formulations and native urine.
- FBS supplementation increased cell death in a dose-dependent manner but remained below 10%.
- The highest TER was observed in cells cultured with AU-Siriraj+2.5% FBS, indicating enhanced cell polarization.
Conclusions:
- Supplementing AU-Siriraj with 2.5% FBS improves renal tubular cell function and polarization.
- AU-Siriraj+2.5% FBS is a suitable medium for in vitro studies of renal cell biology and function, excluding secretome analysis.
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