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Designing a Bio-responsive Robot from DNA Origami
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Aptamer-Integrated Scaffolds for Biologically Functional DNA Origami Structures.

Xiaoxing Chen1,2, Bin Jia1,2, Zhangwei Lu1,2

  • 1Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu 210023, P. R. China.

ACS Applied Materials & Interfaces
|August 17, 2021
PubMed
Summary

This study introduces a new method for creating DNA origami nanostructures with built-in aptamers. These functional nanostructures show improved stability and binding efficiency for biomedical applications.

Keywords:
DNA origami scaffoldaptamercustomized scaffoldfunctional DNA nanostructureself-assembly

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Molecular Biology

Background:

  • DNA origami nanostructures are significant for biomedical applications.
  • Highly ordered functional motifs are crucial for their efficacy.
  • Current methods for functionalizing DNA origami can be limiting.

Purpose of the Study:

  • To develop a robust strategy for producing DNA origami scaffolds with integrated aptamer sequences.
  • To enable direct construction of functional DNA origami structures with user-defined aptamer placement.
  • To enhance the stability and functionality of DNA origami for biomedical use.

Main Methods:

  • Customized DNA scaffolds with integrated aptamer sequences were produced.
  • Two different thrombin aptamer sequences were simultaneously inserted into the M13mp18 phage genome.
  • A scaffold integrated with the platelet-derived growth factor aptamer was also created.

Main Results:

  • Aptamers were efficiently and stably integrated in user-defined positions.
  • DNA origami structures showed increased binding efficiency to thrombin.
  • Structures exhibited over 10-fold greater resistance to exonuclease degradation compared to traditional methods.
  • DNA origami structures demonstrated significant inhibitory effects on breast cancer cells (MDA-MB-231).

Conclusions:

  • This scalable method allows for the creation of design-specific scaffolds.
  • It enables the construction of more stable and functionally robust DNA origami structures.
  • This approach provides a foundation for broader applications of DNA origami in medicine.