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Updated: Oct 23, 2025
Generation of Organ-conditioned Media and Applications for Studying Organ-specific Influences on Breast Cancer Metastatic Behavior
Published on: June 13, 2016
Tumor Targeting by Conditioned Medium Derived From Human Amniotic Membrane: New Insight in Breast Cancer Therapy
Ameneh Jafari1, Mostafa Rezaei-Tavirani1, Hassan Niknejad2
1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Objectives:
Traditional breast cancer treatments have challenges including inefficiency, multidrug resistance, severe side effects, and targeting non-specifically. The development of alternative treatment strategies has attracted a great deal of interest. Using the amniotic membrane has become a promising and convenient new approach for cancer therapy. This study aimed to evaluate the anti-cancer ability of conditioned medium extracted from the human amniotic membrane (hAM-CM) on breast cancer cells.
Methods:
Conditioned medium was collected after 48 h incubation of hAM in epithelial up manner. MTT, cell cycle, apoptosis, colony formation, and sphere assays were used to determine the impact of hAM-CM on breast cancer cell lines. The effects of hAM-CM on the migration and invasion of breast cancer cells were determined using scratch wound healing and transwell assays, respectively.
Results:
Based on the results, cell viability was significantly decreased by hAM-CM in a dose-dependent manner. The hAM-CM remarkably induced apoptosis and necrosis of cancer cells. Moreover, cell migration and invasion potential of cancer cells decreased after the hAM-CM treatment. Further, both the number of colonies and their morphologies were affected by the treatment. In the treated group, a significant decrease in the number of colonies along with an obvious change in their morphologies from holoclone shape to a dominant paracolone structure was observed.
Conclusion:
Our results indicate that the conditioned medium derived from the human amniotic membrane able to inhibit proliferation and metastasis of tumor cells and can be considered a natural and valuable candidate for breast cancer therapy.
Insights
Human amniotic membrane conditioned medium (hAM-CM) shows significant anti-cancer effects. This natural therapy inhibits breast cancer cell proliferation, migration, and invasion, offering a promising alternative treatment.
Area of Science:
- Biomedical research
- Cancer biology
- Regenerative medicine
Background:
- Traditional breast cancer treatments face challenges like drug resistance and severe side effects.
- Novel therapeutic strategies are crucial for effective cancer management.
- The human amniotic membrane (hAM) presents a potential source for new anti-cancer agents.
Purpose of the Study:
- To investigate the anti-cancer properties of conditioned medium from the human amniotic membrane (hAM-CM) on breast cancer cells.
- To evaluate the impact of hAM-CM on breast cancer cell viability, proliferation, apoptosis, migration, and invasion.
Main Methods:
- Conditioned medium (hAM-CM) was prepared from human amniotic membrane cultures.
- MTT, cell cycle, apoptosis, colony formation, and sphere assays were performed on breast cancer cell lines.
- Scratch wound healing and Transwell assays assessed cell migration and invasion.
Main Results:
- hAM-CM significantly reduced breast cancer cell viability in a dose-dependent manner.
- hAM-CM induced apoptosis and necrosis, and inhibited cell migration and invasion.
- Colony formation was suppressed, with morphological changes observed in treated cells.
Conclusions:
- Conditioned medium from the human amniotic membrane demonstrates potent anti-proliferative and anti-metastatic effects on breast cancer cells.
- hAM-CM is a promising natural candidate for developing novel breast cancer therapies.
- Further research into hAM-CM could lead to innovative and less toxic cancer treatments.
