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Updated: Oct 23, 2025
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Published on: September 25, 2019
Generation of a homozygous ZBTB7A knockout human induced pluripotent stem line by CRISPR/Cas9 editing
Diyu Chen1, Bing Song1, Yi Cheng1
1Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China; Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
Abstract:
ZBTB7A plays important roles in several biological processes, including silencing of the fetal γ-globin genes, hematopoiesis, primed-to-naive transition, etc. Meanwhile, it is also associated with Oncogenic transformation and tumor progression. However, the mechanism of ZBTB7A function is not fully understood yet. Here, we generated a homozygous ZBTB7A knockout human induced pluripotent stem cell (iPSC) line, GZHMCi007-A by the CRISPR/Cas9-mediated homology-dependent DNA repair method. The iPSCs of ZBTB7A-/- established by us is a powerful tool for related research.
Insights
Researchers created a ZBTB7A knockout human induced pluripotent stem cell (iPSC) line. This ZBTB7A-null iPSC line is a valuable tool for studying ZBTB7A
Area of Science:
- Genetics
- Stem Cell Biology
- Cancer Biology
Background:
- ZBTB7A is implicated in critical biological processes like gene silencing and hematopoiesis.
- ZBTB7A also plays a role in oncogenic transformation and tumor progression.
- The precise mechanisms of ZBTB7A's function remain incompletely understood.
Purpose of the Study:
- To generate a ZBTB7A knockout human induced pluripotent stem cell (iPSC) line.
- To provide a research tool for investigating ZBTB7A's biological functions and mechanisms.
Main Methods:
- CRISPR/Cas9-mediated homology-dependent DNA repair.
- Generation of a homozygous ZBTB7A knockout (ZBTB7A-/-) human iPSC line (GZHMCi007-A).
Main Results:
- Successfully established a homozygous ZBTB7A knockout human iPSC line, designated GZHMCi007-A.
- The generated iPSC line is characterized by the complete absence of ZBTB7A expression.
Conclusions:
- The ZBTB7A-/- iPSC line is a robust model for studying ZBTB7A's role in various biological contexts.
- This tool will facilitate research into ZBTB7A's involvement in development, hematopoiesis, and cancer.
